Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1969821 | Clinical Biochemistry | 2008 | 4 Pages |
Abstract
ObjectiveCompare evaporation techniques in the work-up procedure of saliva samples for cortisol analysis by a previously validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) method.Design and methodsSamples were evaporated using freeze drying, centrifugal concentration, and nitrogen flow and analyzed by LC–MS/MS.ResultsCortisol concentrations detected using each technique did not significantly differ. Factors other than the recovery of cortisol were evaluated.ConclusionsEvaporation using freeze drying/centrifugal concentration is an automated process, reducing overall processing time for analysis of numerous samples.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Elizabeth A. Nelson, Enzo A. Palombo, Simon R. Knowles,