| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1970169 | Clinical Biochemistry | 2007 | 4 Pages |
Abstract
Objectives:To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.Design and methods:Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.Results:Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively.Conclusions:Norfloxacin method is a simple, inexpensive and automatable kinetic assay.
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Authors
Babu Lal Somani, Vivek Ambade,