Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood

Article ID	Journal	Published Year	Pages	File Type
1970472	Clinical Biochemistry	2007	8 Pages	PDF

Abstract

We developed an internally standardized, specific and reproducible real-time RT-PCR analysis method for PSA and hK2 mRNA in circulating cells in the bloodstream. Both PSA and hK2 assays are sufficiently sensitive to detect two LNCaP cells per milliliter whole blood.

Keywords

PSA qRT-PCR HK2 reproducibility of results Sensitivity and specificity neoplasm circulating cells reverse transcriptase polymerase chain reaction real-time quantitative reverse transcription polymerase chain reaction prostate-specific antigen Tissue kallikreins

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

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Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood

Authors

Maria Rissanen, Pauliina Helo, Riina-Minna Väänänen, Veikko Wahlroos, Hans Lilja, Martti Nurmi, Kim Pettersson, Jussi Nurmi,