| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1970555 | Clinical Biochemistry | 2006 | 4 Pages |
Abstract
ObjectivesGenetic typing of the intronic (CA)n loci within the dystrophin gene was exploited for linkage analysis and diagnosis of carrier status.Design and methodsIP-RP-HPLC was developed for genotyping of 98 chromosomes.ResultsThe typing of 2-bp STRs with mean SD of ± 0.43 bp ensured an allele's identity with 99.7% confidence.ConclusionThe automation enabled the accurate sizing of unlabeled alleles with an excellent reproducibility of 100% with significant labor saving and reduced reagent costs.
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Authors
Nasser Attia Elhawary, Rabah Mohamed Shawky, Nermine Elsayed,