HDL-associated enzymes and proteins in hemodialysis patients

ObjectivesTo evaluate HDL-associated proteins and enzymes and their relation with lipoprotein profile and inflammatory markers in chronic renal patients on hemodialysis.Design and methodsWe studied 53 patients under hemodialysis and 32 healthy subjects as controls. We compared plasma lipids, Apoprotein-AI and hs-CRP, as a marker of chronic inflammation. We evaluated proteins and enzymes associated to HDL, involved in several points of lipoprotein metabolism: CETP, paraoxonase and LpPLA2 activities. Hepatic lipase was measured in postheparin plasma.ResultsPatients showed higher triglycerides and lower LDL-, HDL- and total-cholesterol than controls (p < 0.05). Also, in comparison with controls, Apoprotein-AI, paraoxonase and hepatic lipase were lower, while CETP was higher (p < 0.03). LpPLA2 did not show changes between groups.ConclusionBeyond plasma lipid-lipoprotein profile, other factors could contribute to induce a pro-oxidative and pro-inflammatory status. The protective role of HDL does not only depend on its concentration, but also on its functionality.

► HDL-associated proteins and enzymes and inflammatory markers in hemodialysis. ► Increase in CRP was associated to the reduction in PON activity in HD. ► LDL particles contain more Lp-PLA2 suggested by the high Lp-PLA2/LDL-chol ratio in HD. ► CETP activity was higher and correlated inversely with the HDL-cholesterol in HD.