Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1970740 | Clinical Biochemistry | 2006 | 7 Pages |
Abstract
An enzymatic colorimetry-based DNA microarray assay was successfully established to monitor HBV mutations. Pre-core/core and BCP mutations of HBV genes could be major causes of HBV infection in HBeAg-negative patients and could also be relevant to chronicity and aggravation of hepatitis B.
Keywords
PCR-Restriction Fragment Length PolymorphismPBSX-GalCHBSDSCTLAMPFHBBCPDTTPCR-SSCPASCIPTGBCIP/NBTBSADNAbovine serum albuminAmpicillindeoxyribonucleic acidisopropyl-beta-d-thiogalactopyranosideGene mutationAsymptomatic carrierdithiothreitolPCR-RFLPDNA microarraysodium dodecyl sulfatephosphate buffer solutionbasic core promoterchronic hepatitis BHBVpolymerase chain reactionPCRhepatitis B virus
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Authors
Hailei Mao, Huimin Wang, Donglei Zhang, Hongju Mao, Jianlong Zhao, Jian Shi, Zhichu Cui,