New reusable Celite®/ethylene glycol cartridges for selective chromatography of steroids before immunoassay

ObjectivesPreparation of reusable and easy to handle Celite® chromatographic columns.Design and methodsWeighting precise Celite® quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite® under negative pressure.ResultsThese new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good.ConclusionsThe reusable Celite® cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite® minicolumns.