| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1971140 | Clinical Biochemistry | 2009 | 5 Pages |
BackgroundThe Sebia Capillarys 2, a capillary electrophoresis method, was compared to a high performance liquid chromatography (HPLC) and electrophoresis at alkaline and acid pH for the presumptive identification and quantitation of hemoglobin variants.MethodsThe concordance of hemoglobin variant identification on the Sebia Capillarys 2 with the combination of HPLC and electrophoresis at alkaline and acid pH was evaluated by analyzing samples on both systems. The quantification, expressed as % of total hemoglobin, of the common hemoglobin variants on the Capillarys 2 and the Bio Rad VARIANT II β thalassemia methods were compared.ResultsThe % hemoglobin variant results for the two methods were similar for HbS and slightly different for HbC and D Punjab and significantly different for HbE. The Sebia Capillarys 2 correctly identified a number of hemoglobin variants.ConclusionThe Sebia Capillarys 2 is suitable for the presumptive identification and quantification of hemoglobin variants producing results comparable with existing HPLC and electrophoresis methods.
