Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1975258 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2014 | 12 Pages |
Abstract
We cloned and characterized cDNA sequence of insulin-like growth factor binding protein-4 (IGFBP-4) from Japanese flounder (Paralichthys olivaceus). The 1493Â bp full-length cDNA sequence contained an open reading frame (ORF) of 780Â bp, which encoded a protein of 259 amino acids. The deduced amino acid sequences included a putative signal peptide of 28 amino acid residues resulting in a mature protein of 231 amino acids. Twenty cysteine residues and two conserved IGFBPs motif (GCGCCXXC and CWCV) were found in the N- and C-terminal domain. In the over 13Â kbp genomic sequence, four exons, three introns, and 5â²-/3â²-flanking sequences were identified. Sequence alignment and phylogenetic analysis showed that Japanese flounder IGFBP-4 was indeed the ortholog of the human IGFBP-4 gene and shared high identities with other teleost IGFBP-4 genes. The promoter region was also analyzed and several potential transcription factor (TF) binding sites were determined which may modulate the IGFBP-4 expression. Quantitative real-time PCR analysis revealed that IGFBP-4 mRNA was observed in various tissues, with intestine showing the highest expression. The maternal transcripts of IGFBP-4 gene existed in the early embryonic stages and then increased in the following stages until hatching, suggesting that IGFBP-4 may be involved in the fish early development. The expression level of IGFBP-4 mRNA was relatively higher at 3Â days post hatching (dph) and 15Â dph, and gradually decreased during the metamorphosis period. All these results indicated that IGFBP-4 plays a significant role in IGF regulating vertebrate growth and development.
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Authors
Jing Wang, Jinning Gao, Wenji Wang, Liman Ma, Mengmeng Liu, Haiyang Yu, Zhigang Wang, Xubo Wang, Jie Qi, Quanqi Zhang,