| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1975372 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2013 | 6 Pages |
The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to feeding in chicks. A total of 18 thirteen day-old male chicks were fasted for 12 h. The mRNA levels of the genes in the liver and white adipose tissue were analyzed after 0, 2, and 4 h of refeeding. The mRNA levels of sterol regulatory element-binding protein (SREBP) 1, liver X receptor α, peroxisome proliferator-activated receptor (PPAR) γ, acetyl-CoA carboxylase α and fatty acid synthase were significantly increased after 2 h of refeeding. In contrast, the mRNA levels of PPARα and carnitine palmitoyltransferase 1a were significantly decreased after 2 h of refeeding. The mRNA level of acyl-CoA oxidase was significantly decreased after 4 h of refeeding. The mRNA levels of cholesterol metabolism-related genes such as SREBP2 and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased after 2 h of refeeding. In the white adipose tissue, the mRNA level of PPARγ was significantly increased after 2 h of refeeding, whereas the mRNA level of adipose triglyceride lipase was significantly decreased after 4 h of refeeding. These results demonstrated that expression of lipid metabolism-related genes is regulated by short-term refeeding in chicks.
