| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1976488 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2009 | 7 Pages |
This study examined ontogeny of development for a range of adipokines in neonatal adipose tissue. Pigs (Sus scrofa) were selected across six litters for sampling subcutaneous (SQ) and perirenal (PR) adipose tissues at d1, d4, d7 or d21 of age and total RNA extraction. Reverse transcription and real-time PCR were used to quantify mRNA abundance for: leptin, adiponectin, interleukin 1β (IL-1β), IL-6, IL-8, IL-10, IL-15, tumor necrosis factor α (TNFα), haptoglobin, vascular endothelial growth factor (VEGF), macrophage migration inhibitory factor (MIF), monocyte chemoattractant protein 1 (MCP1) and cyclophilin. Leptin, adiponectin and IL-15 expression increased from d1 to d 21 of age in both SQ and PR. Haptoglobin, VEGF, MIF and IL-8 expression decreased between d1 and d4 of age in SQ. TNFα expression was unchanged from d1–7 and then increased at d21. IL-1β, IL-6 and IL-10 expression were unchanged with age in SQ; whereas IL-1β and IL-6 mRNA abundance in the PR increased with age. Analysis of the mRNA abundance for these adipokines within adipose tissue from d1 to d21 of age demonstrated that neonatal development of adipokine expression varies among the different adipokines and the internal and external sites of adipose tissue deposition (PR versus SQ).
