Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1977594 | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | 2008 | 6 Pages |
Abstract
The contribution of antibodies directed against the two main toxic groups of proteins in the Vipera ammodytes ammodytes venom, haemorrhagic metalloproteinases (H) and neurotoxic sPLA2s (Atxs), to the overall protective efficacy of the whole venom antisera was investigated. Using ELISA assays we established a high correlation between the protective efficacy of the whole venom antisera in mice and their anti-Atxs antibody content. As the haemorrhage is the prevailing toxic effect of the venom in human, the lack of correlation also with anti-H IgG content exposed that the mouse model might not be optimal to evaluate the neutralizing potential of the venom-specific antisera for human therapy. We further revealed that Atxs and structurally very similar but non-toxic AtnI2 from the venom are not immuno cross-reactive.
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Authors
Beata Halassy, Lidija Habjanec, Marija Brgles, Maja Lang Balija, Adrijana Leonardi, Lidija KovaÄiÄ, Petra Prijatelj, Jelka TomaÅ¡iÄ, Igor Križaj,