Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1978368 | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | 2007 | 6 Pages |
Abstract
In the present study, the bioaccumulation and reactive oxygen species (ROS) generation were studied after fish (Carassius auratus) were exposed to different concentrations (0.01, 0.02, 0.05, 0.07 and 0.1 mg/L) of phenanthrene for 4 days. The accumulation of phenanthrene in liver increased with the exposure concentration (R2 = 0.88). A secondary spin trapping technique was used followed by electron paramagnetic resonance (EPR) analysis, to study the ROS production. The ROS generated in fish liver after exposure to phenanthrene was identified as hydroxyl radical (OH). The OH signal intensity of the EPR spectrum showed a significant increase (p < 0.05) compared to the control when the phenanthrene concentration was as low as 0.05 mg/L. A good positive relationship (R2 = 0.97) was found between the OH formation and exposure concentrations. The changes of the activities of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), and contents of reduced glutathione (GSH) also were detected. The results clearly indicated that phenanthrene could induce OH generation and result in oxidative stress in liver of fish.
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Authors
Ying Yin, Haixia Jia, Yuanyuan Sun, Hongxia Yu, Xiaorong Wang, Jichun Wu, Yuqun Xue,