Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1979268 | Current Opinion in Structural Biology | 2010 | 9 Pages |
Abstract
While we appreciate the complexity of the intracellular environment as a general property of every living organism, we collectively lack the appropriate tools to analyze protein structures in a cellular context. In-cell NMR spectroscopy represents a novel biophysical tool to investigate the conformational and functional characteristics of biomolecules at the atomic level inside live cells. Here, we review recent in-cell NMR developments and provide an outlook towards future applications in prokaryotic and eukaryotic cells. We hope to thereby emphasize the usefulness of in-cell NMR techniques for cellular studies of complex biological processes and for structural analyses in native environments.
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Authors
Yutaka Ito, Philipp Selenko,