High throughput platforms for structural genomics of integral membrane proteins

Structural genomics approaches on integral membrane proteins have been postulated for over a decade, yet specific efforts are lagging years behind their soluble counterparts. Indeed, high throughput methodologies for production and characterization of prokaryotic integral membrane proteins are only now emerging, while large-scale efforts for eukaryotic ones are still in their infancy. Presented here is a review of recent literature on actively ongoing structural genomics of membrane protein initiatives, with a focus on those aimed at implementing interesting techniques aimed at increasing our rate of success for this class of macromolecules.

► Structural genomics techniques have been developed for membrane proteins. ► Structures of prokaryotic and eukaryotic membrane proteins can be determined. ► High throughput methods increase chances of finding a ‘well-behaved’ membrane protein.