| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1980682 | DNA Repair | 2011 | 9 Pages |
Abstract
⺠Processivity of DNA glycosylases can cause double-strand breaks at clustered lesions. ⺠We engineered pyrimidine dimer-specific DNA glycosylases with reduced processivity. ⺠We developed an assay to quantitatively measure incisions at clustered abasic sites. ⺠Biochemical and computational data reveal residues critical for processive nicking. ⺠Enzymes with reduced processivity but full catalytic function can be engineered.
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Authors
Olga P. Ryabinina, Irina G. Minko, Michael R. Lasarev, Amanda K. McCullough, R. Stephen Lloyd,