Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1981596 | FEBS Open Bio | 2015 | 9 Pages |
Abstract
The wound healing potency of an aqueous extract of placenta can be evaluated through the presence of numerous regulatory components. The presence of glycans was detected by thin layer chromatography and fluorophore-assisted carbohydrate electrophoresis. Mass spectrometric analysis revealed the existence of multiple fragments of immunoglobulin G (IgG). IgG was present in the extract at a concentration of 25.2 ± 3.97 μg/ml. IgG possesses anti-complementary activity by diverting the complement activation from target surface. Thus, effect of placental IgG on complement-bacteria interaction was investigated through classical and alternative pathway and the preparation was ascertained to be safe with respect to their interference in the process of bacterial opsonization.
Keywords
IgGEGTAPNGase FCNBrNBTnitro-blue tetrazolium chlorideBHIG6PDHBCIPBSANAD+Adenosine TriphosphateATPbovine serum albuminEDTAethylene glycol tetra acetic acidethylenediamine tetra acetic acidimmunoglobulin immunoglobulin GBrain–Heart infusionHuman placental extractComplementAntsNAD, nicotinamide adenine dinucleotidepeptide N-glycosidase Fglucose-6-phosphate dehydrogenase
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Authors
Kanika Sharma, Debasish Bhattacharyya,