Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1981610 | FEBS Open Bio | 2015 | 10 Pages |
•Oxidative stress in the arthritis joint is involved in generating mediators for inflammation.•Oxidative stress-induced expression of Cox-2 was mediated by MAPKs and NF-κB.•ROS-induced MAPKs and NF-κB were attenuated by inhibition of MAPKKK TAK1.•Inhibition of TAK1 activity resulted in reduced expression of Cox-2 and PGE2.•ROS-induced TAK1 activation and Cox-2 expression was inhibited by antioxidants N-acetyl cysteamine and hyaluronic acid.
Oxidative stress within the arthritis joint has been indicated to be involved in generating mediators for tissue degeneration and inflammation. COX-2 is a mediator in inflammatory action, pain and some catabolic reactions in inflamed tissues. Here, we demonstrated a direct relationship between oxidative stress and Cox-2 expression in the bovine synovial fibroblasts. Furthermore, we elucidated a novel mechanism, in which oxidative stress induced phosphorylation of MAPKs and NF-κB through TAK1 activation and resulted in increased Cox-2 and prostaglandin E2 expression. Finally, we demonstrated that ROS-induced Cox-2 expression was inhibited by supplementation of an antioxidant such as N-acetyl cysteamine and hyaluronic acid in vitro and in vivo. From these results, we conclude that oxidative stress is an important factor for generation of Cox-2 in synovial fibroblasts and thus its neutralization may be an effective strategy in palliative therapy for chronic joint diseases.