Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1983336 | The International Journal of Biochemistry & Cell Biology | 2016 | 8 Pages |
Abstract
As electromagnetic field (EMF) is commonly encountered within our daily lives, the biological effects of EMF are of great concern. Autophagy is a key process for maintaining cellular homeostasis, and it can also reveal cellular responses to environmental stimuli. In this study, we aim to investigate the biological effects of a 50 Hz-sinusoidal electromagnetic field on autophagy and we identified its mechanism of action in Chinese Hamster Lung (CHL) cells. CHL cells were exposed to a 50 Hz sinusoidal EMF at 0.4 mT for 30 min or 24 h. In this study, we found that a 0.4 mT EMF resulted in: (i) an increase in LC3-II expression and increased autophagosome formation; (ii) no significant difference in the incidence of γH2AX foci between the sham and exposure groups; (iii) reorganized actin filaments and increased pseudopodial extensions without promoting cell migration; and (iv) enhanced cell apoptosis when autophagy was blocked by Bafilomycin A1. These results implied that DNA damage was not directly involved in the autophagy induced by a 0.4 mT 50 Hz EMF. In addition, an EMF induced autophagy balanced the cellular homeostasis to protect the cells from severe adverse biological consequences.
Keywords
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Authors
Yunyun Shen, Ruohong Xia, Hengjun Jiang, Yanfeng Chen, Ling Hong, Yunxian Yu, Zhengping Xu, Qunli Zeng,