Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1984182 | The International Journal of Biochemistry & Cell Biology | 2009 | 7 Pages |
Abstract
Bax inhibitor-1 is a conserved protein which suppresses endoplasmic reticulum stress-induced apoptosis and regulates calcium release from the endoplasmic reticulum. Recent studies have revealed that adipogenesis, the process of adipocyte differentiation, is influenced by a change of intracellular calcium concentration. Here, we examined the effect of endoplasmic reticulum calcium regulation by Bax inhibitor-1 on adipogenesis using 3T3-L1 preadipocytes stably transfected with a pcDNA3-BI-1-HA plasmid. Bax inhibitor-1 functionality was confirmed by inhibiting the thapsigargin-induced increase of endoplasmic reticulum stress markers. Bax inhibitor-1 overexpression did not alter normal process of adipogenesis. Thapsigargin treatment inhibited adipogenesis in control cells, but Bax inhibitor-1 overexpressing 3T3-L1 cells retained their adipogenesis function. Endoplasmic reticulum stress did not seem to be involved in thapsigargin-reduced adipogenesis, since other endoplasmic reticulum stress inducers, such as tunicamycin and dithiothreitol, did not suppress the differentiation of 3T3-L1 cells. Bax inhibitor-1 might affect adipogenesis through regulating cytosolic calcium, because the thapsigargin-induced robust intracellular calcium rise was not observed in Bax inhibitor-1 overexpressing 3T3-L1 cells. A23187, a calcium ionophore, showed the same effect on adipogenesis as thapsigargin. Taken together, Bax inhibitor-1 overexpression in 3T3-L1 preadipocytes inhibits calcium mobilizing agent-induced suppression of adipogenesis. As adipogenesis is dependent on a change of intracellular calcium concentration, endoplasmic reticulum calcium regulation by Bax inhibitor-1 may play an important role in adipogenesis process.
Keywords
PPARγGRP78AP2ThapsigargineIF2αXBP-1BI-1Pref-1Sarco-endoplasmic reticulum Ca2+-ATPaseTUNLPLGAPDHtunicamycinFASpreadipocyte factor-1DTTSREBPAdipocyteEndoplasmic reticulum stressfatty acid synthaseTHAPTriacylglycerideDifferentiationX-box binding protein-1dithiothreitolendoplasmic reticulumeukaryotic translation initiation factor 2αSERCALipoprotein lipasebax inhibitor-1hemagglutininsterol response element binding protein78 kDa glucose-regulated proteinIntracellular calciumglyceraldehyde-3-phosphate dehydrogenaseperoxisome proliferator-activated receptor γ
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Authors
Jiahua Yu, Kang-Beom Kwon, Jin-Woo Park, Han-Jung Chae, Byung-Hyun Park,