| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1985691 | International Journal of Biological Macromolecules | 2016 | 10 Pages |
•Synthesized a novel drug carrier for simultaneous glucose sensing and insulin delivery.•Prepared material facilitates efficient entrapment of insulin owing to the increased hydrophobicity of beta-cyclodextrin.•The biological effectiveness was assessed using flow cytometry analysis.•Prepared material can act as a potential substitute for conventional forms of insulin administration.
Inconsistent dosage of insulin (INS) for type 2 diabetes patients lead to severe adverse effects like limb amputation, blindness and fatal hypo or hyper glycaemia. Hence, a drug delivery system (DDS) capable of consistent INS release by sensing changes in blood glucose level is essential. Herein, we report a glucose responsive DDS comprised of oleic acid-grafted-aminated beta cyclodextrin (OA-g-ACD) copolymer, coated with a dispersion of glucose oxidase (GOx) and catalase (CAT). The prepared DDS was characterised using FTIR, Optical Microscopy, H1 NMR, DLS and SEM. Hydrophobicity and drug loading capacity was ascertained using contact angle measurements and confocal laser scanning microscopy (CLSM) respectively. Extent of swelling was observed to be a function of glucose concentration. INS release profile showed a cumulative release of 78.0 % after 240 min. Flow cytometry studies revealed greater population of INS on HeLa cells indicating application of DDS as potential candidate for the intravenous administration of INS.
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