Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1986109 | International Journal of Biological Macromolecules | 2016 | 10 Pages |
•Synthesized quinapyramine sulfate loaded-sodium alginate nanoparticles (QS-NPs) using emulsion cross-linking method.•Determined the safety of the formulated nanoparticles, using Vero, Hela cell lines and horse erythrocytes in a dose-dependent manner.•Unveiled concentration-dependent cytotoxicity, genotoxicity, production of reactive oxygen species and hemolysis in QS-NPs treated cells.•QS-NPs were safe at effective trypanocidal doses and even at doses several times higher than the effective dose.
We synthesized quinapyramine sulfate loaded-sodium alginate nanoparticles (QS-NPs) to reduce undesirable toxic effects of QS against the parasite Trypanosoma evansi, a causative agent of trypanosomosis. To determine the safety of the formulated nanoparticles, biocompatibility of QS-NPs was determined using Vero, Hela cell lines and horse erythrocytes in a dose-dependent manner. Our experiments unveiled a concentration-dependent safety/cytotoxicity (metabolic activity), genotoxicity (DNA damage, chromosomal aberrations), production of reactive oxygen species and hemolysis in QS-NPs treated cells. Annexin-V propidium iodide (PI) staining showed no massive apoptosis or necrosis. However, at very high doses (more than 300 times than the effective doses), we observed more toxicity in QS-NPs treated cells as compared to QS treated cells. QS-NPs were safe at effective trypanocidal doses and even at doses several times higher than the effective dose.