Microbial production of four biodegradable siderophores under submerged fermentation

•Four siderophore analogues were isolated, purified and well characterized from Escherichia coli, Bacillus spp. ST13, and Streptomyces pilosus microorganisms under some specific submerged fermentation conditions.•The four isolated and purified siderophore analogues were found to be biodegradable.•The cytotoxic activities of those siderophore bioligands and their ferric complexes were tested and evaluated against tumor cell lines.•The antioxidant activities of those siderophore bioligands and their ferric complexes were examined by free radical scavenging assay.•The anti-inflammatory activities of these biodegradable siderophore bioligands and their ferric complexes were tested and evaluated.

Four siderophore analogues were isolated and purified from Escherichia coli, Bacillus spp. ST13, and Streptomyces pilosus microorganisms under some specific submerged fermentation conditions. In order to evaluate the highest production of this siderophore analogues through the growth, a rapid spectrophotometric screening semi-quantitative method was used, in which interestingly the analogues were isolated in its own form not its iron chelate. After chromatographic separation, the chemical structures of the isolated and purified siderophores were illustrated using detailed spectroscopic techniques. The biodegradation studies were done on that four novel isolated and purified siderophores following OECD protocols. In addition, the bioactivities of these siderophores and their iron complexes were examined and evaluated.

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