Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1986260 | International Journal of Biological Macromolecules | 2015 | 6 Pages |
Abstract
A fructan designated as CKNP with apparent molecular weight of 5.3 kD was isolated from the hot water extract of Curcuma kwangsiensis through a combination of ion-exchange chromatography on DEAE 650 M and gel filtration on Superdex G-200. CKNP was characterized by chemical derivatization as well as HPLC, GC, and GC-MS technologies. Structural studies revealed that CKNP is composed predominately of fructose (96.8%) and a small amount of glucose (3.2%) with a degree of polymerization (DP) of 30-31. It was deduced to be a levan-type fructan containing a backbone composed of (2 â 6)-linked β-d-Fruf residues and single β-d-Fruf residues as side chains branched at the O-1 position along the backbone. Preliminary in vitro bioactive tests on RAW 264.7 murine macrophage cells revealed that the levan-type fructan from C. kwangsiensis shows significant immunostimulating activity based on its ability to stimulate macrophage proliferation and enhance phagocytosis.
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Authors
Cai-Xia Dong, Li-Jiao Zhang, Rong Xu, Gong Zhang, Ying-Biao Zhou, Xiao-Qiang Han, Ying Zhang, Yuan-Xia Sun,