Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1987207 | International Journal of Biological Macromolecules | 2012 | 10 Pages |
Abstract
Laser desorption of dye-tagged oligonucleotides was studied using laser-induced fluorescence imaging. Desorption with ultra violet (UV) and infra-red (IR) lasers resulted in forward directed plumes of molecules. In the case of UV desorption, the initial shot desorbed approximately seven-fold more material than subsequent shots. In contrast, the initial shot in IR desorption resulted in the ejection of less material compared to subsequent shots and these plumes had a component directed along the path of the laser. Thermal equilibrium of the molecules in the plume was achieved after approximately 25 μs with a spread in molecular temperature which was described by a modified Maxwell–Boltzmann equation.
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Authors
Tony L. Merrigan, David J. Timson, C. Adam Hunniford, Martin Catney, Robert W. McCullough,