Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1987569 | International Journal of Biological Macromolecules | 2010 | 5 Pages |
Abstract
Extraction of total DNA from a given habitat assumes significance in metagenomics, due to the requirement of inhibitor free and high quality metagenome in good quantity for applications in molecular biology. DNA extraction and its quality assessment for PCR applications from saline soils of Coastal Gujarat and Sambhar Soda Lake, Rajasthan in India is described in a comparative manner. The mechanical and soft lysis methods were simple and efficient for rapid isolation of PCR amplifiable total genomic DNA. The results are significant as only few extreme environments, particularly saline habitats are explored for their metagenomic potential.
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Authors
P.K. Siddhapura, S. Vanparia, M.K. Purohit, S.P. Singh,