Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1987587 | International Journal of Biological Macromolecules | 2010 | 5 Pages |
Abstract
This paper describes cloning, expression, characterization and crystallization of feruloyl esterase from Aspergillus awamori. The gene coding for feruloyl esterase from A. awamori was amplified, cloned, and expressed in Escherichia coli. The recombinant enzyme was purified, characterized and used for crystallization. Its X-ray crystallographic data were collected and explained using molecular replacement theory. Also, the tertiary structure of the recombinant enzyme–ferulic acid complex was also determined.
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Authors
Ahmed E. Fazary, Hamed A. Hamad, John C. Lee, Takuya Koskei, Cheng-Kang Lee, Yi-Hsu Ju,