| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1988099 | International Journal of Biological Macromolecules | 2006 | 7 Pages |
Abstract
Cell extract gelled upon freezing/thawing and boiling. Additionally, proteins aggregated on various chromatography media upon concentration and during desalting. The polysaccharides clogged the matrix of chromatographic materials and the pores of ultrafiltration membranes. The precipitation of proteins and polysaccharides caused very low resolution and streaking on SDS- and two-dimensional polyacrylamide gels. The present work describes the potential causes for gel formation in anammox cell extracts. Optimized protocols for sample preparation for polyacrylamide gel electrophoresis and ion exchange chromatography are presented. High-resolution gel electrophoresis of the cell extract was achieved after clarification from polymeric substances with denaturating phenol extraction and the purification of a 10Â kDa cytochrome c is presented as an example.
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Authors
Irina E.Y. Cirpus, Wim Geerts, John H.M. Hermans, Huub J.M. Op den Camp, Marc Strous, J. Gijs Kuenen, Mike S.M. Jetten,