Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1988200 | International Journal of Biological Macromolecules | 2008 | 8 Pages |
Abstract
Cyclic AMP serves as an intracellular messenger in cells and regulates a variety of biological functions by transmitting information through proteins. These proteins of different functions all consist of a cAMP-binding motif, and the structure of this motif is highly conserved with an exception of the loop 3 and 4. In current study, cAMP receptor protein was employed as a model system to investigate the function of the two loops. The results indicated that the loop 3 involves in the intersubunits communication of CRP, whereas the loop 4 involves in cAMP binding and interdomains communication.
Keywords
pKaCyclic nucleotide-gated ion channelscNMPCNGCEpacPMSFPAGEITCIPTGCPM3′,5′-cyclic adenosine monophosphatecAMPCommunicationpolyacrylamide gel electrophoresisFluorescence anisotropyThermodynamicsphenylmethylsulfonyl fluoridewild typeExchange protein directly activated by cAMPCRPprotein kinase AcAMP receptor proteinisothermal titration calorimeter
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Authors
Xianrong Shen, Wenbing Tao, Jilie Kong, Shaoning Yu,