Article ID Journal Published Year Pages File Type
1988463 Journal of Biochemical and Biophysical Methods 2007 5 Pages PDF
Abstract

Anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from Trichoderma harzianum ETS 323. The fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. Structure of chrysophanol was solved by X-ray diffraction and pachybasin by NMR spectra. About 233 ± 13 mg of pure chrysophanol and 773 ± 40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7 ± 0.2% and 5.6 ± 0.5%, respectively.

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