Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1988467 | Journal of Biochemical and Biophysical Methods | 2007 | 7 Pages |
Abstract
A novel mixed-ligand nickel(II) complex that contains 1-methylimidazole and thiocyanate, Ni(NCS)2(Mim)4 (Mim = 1-methylimidazole), was synthesized and its structure was determined by X-ray crystallography, IR spectrum and elemental analysis, etc. Its DNA-binding properties were studied by electronic absorption spectral, viscositive and electrochemical measurements. The absorption spectral and viscositive results suggest that the nickel(II) complex binds to DNA via partial intercalation. The addition of DNA results in the decrease of the peak current of the nickel(II) complex proved their interaction. The slight differences of peak profiles and electrochemical parameters between free and DNA-bound Ni(NCS)2(Mim)4 showed the formation of an electrochemical inactive complex between Ni(NCS)2(Mim)4 and DNA. The binding site and binding constant of the complex to DNA were determined by electrochemical titration method.
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Authors
Qing-Xiang Wang, Kui Jiao, Fa-Qian Liu, Xian-Long Yuan, Wei Sun,