| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1988469 | Journal of Biochemical and Biophysical Methods | 2007 | 4 Pages |
Abstract
Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.
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Authors
Joseph A. Price III,