| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1988599 | Journal of Biochemical and Biophysical Methods | 2007 | 6 Pages |
Abstract
Based on the monolithic silica gel materials with hierarchical pore structure and on the SPE devices (MonoTip®) developed thereof, a trypsin-immobilized monolithic silica in a pipette tip (MonoTip® Trypsin) suitable for digesting proteins has been newly developed. The surface of monolithic silica fixed into the tip was chemically modified with trypsin via an aminopropyl group. Trypsin-immobilized monolith successfully performed a rapid digestion of reduced and alkylated proteins with only a few times pipetting operation for the pre-treatment procedure of chromatographic analysis. The novel solid-phase digestion tool using monolithic silica allows a high-throughput trypsin proteolysis of bio-substances in proteomics.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Shigenori Ota, Shota Miyazaki, Hideo Matsuoka, Kei Morisato, Yukihiro Shintani, Kazuki Nakanishi,