| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1988625 | Journal of Biochemical and Biophysical Methods | 2006 | 5 Pages |
Abstract
Representative extraction of both RNA and protein from a single biological sample is required for reliable assessment of coordinated changes in gene and protein expression. Such a simultaneous extraction can be performed by using Trizol® Reagent. Here, we demonstrate that, as an alternative to SDS, 2% diethylamine is an effective solvent, which can be conveniently used in extraction of Trizol®-isolated proteins from various tissues. Diethylamine provides efficient extraction of proteins and compatibility with a variety of common downstream analytical applications.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Rebecca L. Nolan, Jan K. Teller,