Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1993456 | Methods | 2014 | 8 Pages |
Abstract
Here we present a radical improvement in oil red O worm staining together with high-throughput image-based phenotyping. The three-step sample preparation method is robust, formaldehyde-free, and inexpensive, and requires only 15Â min of hands-on time to process a 96-well plate. Together with our free and user-friendly automated image analysis package, this method enables C. elegans sample preparation and phenotype scoring at a scale that is compatible with genome-wide screens. Thus we present a feasible approach to small-scale phenotyping and large-scale screening for genetic and/or chemical perturbations that lead to alterations in fat quantity and distribution in whole animals.
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Authors
Carolina Wählby, Annie Lee Conery, Mark-Anthony Bray, Lee Kamentsky, Jonah Larkins-Ford, Katherine L. Sokolnicki, Matthew Veneskey, Kerry Michaels, Anne E. Carpenter, Eyleen J. O'Rourke,