| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1993521 | Methods | 2012 | 8 Pages |
Interest in the application of advanced proteomics technologies to human blood plasma- or serum-based clinical samples for the purpose of discovering disease biomarkers continues to grow; however, the enormous dynamic range of protein concentrations in these types of samples (often >10 orders of magnitude) represents a significant analytical challenge, particularly for detecting low-abundance candidate biomarkers. In response, immunoaffinity separation methods for depleting multiple high- and moderate-abundance proteins have become key tools for enriching low-abundance proteins and enhancing detection of these proteins in plasma proteomics. Herein, we describe IgY14 and tandem IgY14-Supermix separation methods for removing 14 high-abundance and up to 60 moderate-abundance proteins, respectively, from human blood plasma and highlight their utility when combined with liquid chromatography–tandem mass spectrometry for interrogating the human plasma proteome.
► Immunoaffinity separations are key tools for enriching low-abundance proteins. ► Methods for IgY14 and SuperMix Immunoaffinity separations are described. ► IgY14 separation removes the top 14 abundant proteins from human plasma. ► Tandem IgY14-SuperMix separations remove up to 60 abundant proteins. ► Proteomics applications of IgY14 and SuperMix separations are highlighted.
