Development of electrochemiluminescence-based singleplex and multiplex assays for the quantification of α-synuclein and other proteins in cerebrospinal fluid

The need for improved diagnostic accuracy and markers of progression in neurodegenerative diseases motivates the identification of objective biomarkers as well as optimized assays for their quantification. Several potential marker candidates for Parkinson’s disease (PD) in cerebrospinal fluid have been identified. These include α-synuclein, a major constituent of the intracellular aggregates. We give a general overview and details of our experience in converting established enzyme-linked immunoabsorbent assays (for α-synuclein and other proteins) onto an electrochemiluminescence-based platform as well as considerations on multiplexing different assays for PD.

► We describe our approach for ECL-based quantification of CSF proteins. ► We give details of our established assay for CSF α-synuclein. ► ECL-based assays are highly sensitive as compared to other quantification methods. ► The assay enables multiplexing of analytes in a single sample.