| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1993769 | Methods | 2011 | 7 Pages |
During their life cycles, viruses typically undergo many transport events throughout the cell. These events depend on a variety of both viral and host proteins and are often not fully understood. Such studies are often complicated by asynchronous infections and the concurrent presence of various viral intermediates in the cells, making it difficult to molecularly define each step. In the case of the herpes simplex virus type 1, the etiological agent of cold sores and many other illnesses, the viral particles undergo an intricate series of transport steps during its life cycle. Upon entry by fusion with a cellular membrane, they travel to the host cell nucleus where the virus replicates and assembles new viral particles. These particles then travel across the two nuclear envelopes and transit through the trans-Golgi network before finally being transported to and released at the cell surface. Though viral components and some host proteins modulating these numerous transport events have been identified, the details of these processes remain to be elucidated. To specifically address how the virus escapes the nucleus, we set up an in vitro model that reproduces the unconventional route used by herpes simplex type 1 virus to leave nuclei. This has not only allowed us to clarify the route of capsid egress of the virus but is now useful to define it at the molecular level.
► We reconstitute herpes simplex virus type 1 (HSV-1) nuclear egress in vitro. ► We establish a method to isolate functional nuclei from infected cells. ► HSV-1 nuclear assay depends on time, temperature and requires both energy and cytosol. ► Our results support the HSV-1 de-envelopment/re-envelopment model of transport.
