| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1993899 | Methods | 2011 | 8 Pages |
Abstract
Current antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient’s cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Tre’s antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2−/−γc−/− mice.
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Authors
Frank Buchholz, Joachim Hauber,