| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1994258 | Methods | 2009 | 9 Pages |
Abstract
Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation—through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.
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Authors
Konstantin Kuznedelov, Konstantin Severinov,