Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1994367 | Methods | 2007 | 8 Pages |
Abstract
The core histone tail domains play important roles in different stages of chromatin condensation. The tails are required for folding nucleosome arrays into secondary chromatin structures such as the â¼30Â nm diameter chromatin fiber and for mediating fiber-fiber interactions important for formation of tertiary chromatin structures. Crosslinking studies have demonstrated that inter-nucleosomal tail-DNA contacts appear in conjunction with salt-induced folding of nucleosome arrays into in higher order chromatin structures. However, since both folding of nucleosome arrays and fiber-fiber interactions take place simultaneously in >2-3Â mM MgCl2 such inter-nucleosome interactions may reflect short range (intra-array) or longer range (inter-array) interactions. Here, we describe a novel technique to specifically identify inter-array interactions mediated by the histone tail domains. In addition, we describe a new method for the preparation of H3/H4 tetramers.
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Authors
Pu-Yeh Kan, Jeffrey J. Hayes,