Article ID Journal Published Year Pages File Type
1994862 Microvascular Research 2013 4 Pages PDF
Abstract

•The plasma d-ROM levels increased in SHRSP after 16 weeks of age.•Cerebral edema was apparent in one SHRSP at 18 weeks of age.•No significant differences were found in 8-OHdG and lipid peroxidation markers.•Changes in plasma and tissue d-ROMs were closely associated with the changes in BBB related factor.

To elucidate the pathogenic roles of oxidative stress on blood–brain-barrier (BBB) dysfunction, we compared the chronological changes of oxidative stress in blood and cerebral tissue between stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar–Kyoto rats (WKY). Plasma and tissue oxidative stress was assayed by the diacron-reactive oxygen metabolite (d-ROM) test using 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a reference oxidative stress marker. The plasma and cerebral cortex d-ROM levels increased in SHRSP after 16 weeks of age, but not in WKY. There were no significant differences in 8-OHdG or lipid peroxidation markers between SHRSP and WKY. Antioxidant capacity, as estimated by the biological antioxidant potential test, was similar between SHRSP and WKY at all ages examined. The changes in plasma and tissue d-ROM levels coincided with changes in glucose transporter-1 and aquaporin-4 expression, as functional constituents of the BBB. These results indicate that plasma oxidative stress increases before the onset of tissue damage, and plays an important role in BBB dysfunction rather than decreases in antioxidant capacity. The plasma d-ROM test appears to be useful for predicting vasogenic cerebral edema in severe hypertension.

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