Myoendothelial gap junction frequency does not account for sex differences in EDHF responses in rat MCA

Membrane potential in endothelium was measured using the voltage-sensitive dye di-8-ANEPPS and in smooth muscle using intracellular glass microelectrodes in the presence of l-NAME (3 × 10− 5 M) and indomethacin (10− 5 M). Electron microscopy was used to assess MEGJ characteristics. In endothelial cells, the di-8-ANEPPS fluorescence ratio change to 10− 5 M UTP was similar in males (− 2.9 ± 0.5%) and females (− 3.2 ± 0.2%), indicating comparable degrees of endothelial cell hyperpolarization. However, smooth muscle cell hyperpolarization to 10− 5 M UTP was significantly attenuated in females (0 mV hyperpolarization; − 31 ± 1.5 mV resting) compared to males (8 mV hyperpolarization; − 28 ± 1.7 mV resting). Ultrastructural evidence suggested that MEGJ frequency and area of contact were comparable between males and females. Taken together, our data suggest that in rat MCA, MEGJ frequency does not account for the reduced EDHF responses observed in females compared to males. We conclude that reduced myoendothelial coupling and/or homocellular coupling within the media may account for these differences.