Clustered Mutations in Yeast and in Human Cancers Can Arise from Damaged Long Single-Strand DNA Regions

► Clusters of simultaneous multiple mutations occur in yeast and human genomes ► Mutation clusters can occur in damaged ssDNA during DSB repair or replication ► Clusters of coordinated C or G mutations in cancers colocalize with rearrangements ► Clustered mutations in cancers occur at motifs of cytosine deamination by APOBECs