Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
19998 | Journal of Bioscience and Bioengineering | 2016 | 5 Pages |
Abstract
Cyclic diguanylate (c-di-GMP) is a second messenger of bacteria and its detection is an important issue in basic and applied microbiology. As c-di-GMP riboswitch ligand-binding domains (aptamer domains) capture c-di-GMP with high affinity and selectivity, they are promising platforms for the development of RNA-based c-di-GMP sensors. We analyzed two previously reported c-di-GMP sensor RNAs derived from the Vc2 riboswitch. We also designed and tested their variants, some of which showed improved properties as RNA-based c-di-GMP sensors.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Saki Inuzuka, Shigeyoshi Matsumura, Yoshiya Ikawa,