Article ID Journal Published Year Pages File Type
2002045 Nitric Oxide 2006 6 Pages PDF
Abstract

The measurement of nitric oxide synthase activity in cell lysates is often performed by radiochemical assay that quantifies the conversion of l-[3H]arginine to l-[3H]citrulline. We have developed a spectrophotometric procedure which continuously recycles NADPH through the addition of glucose 6-phosphate dehydrogenase to the cell lysate. This allows nitric oxide synthase to operate linearly for hours, so that nitric oxide-derived nitrite accumulates at amounts sufficient to be detected with the Griess assay. The incorporation of cycling of NADPH also improves the radiochemical assay for nitric oxide synthase activity.

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