Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2002045 | Nitric Oxide | 2006 | 6 Pages |
Abstract
The measurement of nitric oxide synthase activity in cell lysates is often performed by radiochemical assay that quantifies the conversion of l-[3H]arginine to l-[3H]citrulline. We have developed a spectrophotometric procedure which continuously recycles NADPH through the addition of glucose 6-phosphate dehydrogenase to the cell lysate. This allows nitric oxide synthase to operate linearly for hours, so that nitric oxide-derived nitrite accumulates at amounts sufficient to be detected with the Griess assay. The incorporation of cycling of NADPH also improves the radiochemical assay for nitric oxide synthase activity.
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Authors
Dario Ghigo, Chiara Riganti, Elena Gazzano, Costanzo Costamagna, Amalia Bosia,