Article ID Journal Published Year Pages File Type
2006148 Peptides 2013 9 Pages PDF
Abstract

•ANP effect on H2O2-induced ROS generation, cell proliferation, migration and cytokine release was detected in monocytes.•ANP was able to increase the H2O2-dependent ROS production partly by the involvement of the NADPH oxidase.•ANP was able to stimulate cell migration and decrease THP-1 proliferation.•ANP plays a role in the IL-9, TNF-α, MIP-1α release whereas no effect was observed for IL-6 and IL-1β.•Our results support the view that ANP can play a key role during the inflammatory process.

Atrial natriuretic peptide (ANP), a cardiovascular hormone, elicits different biological actions in the immune system. The aim of the present study was to investigate in THP-1 monocytes the ANP effect on hydrogen peroxide (H2O2)-induced Reactive Oxygen Species (ROS), cell proliferation and migration. A significant increase of H2O2-dependent ROS production was induced by physiological concentration of ANP (10−10 M). The ANP action was partially affected by cell pretreatment with PD98059, an inhibitor of mitogen activated-protein kinases (MAPK) as well as by wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K) and totally suppressed by diphenylene iodonium (DPI), an inhibitor of the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. The hormone effect was mimicked by cANF and an ANP/NPR-C signaling pathway was studied using pertussis toxin (PTX). A significant increase of H2O2-induced cell migration was observed after ANP (10−10 M) treatment, conversely a decrease of THP-1 proliferation, due to cell death, was found. Both ANP actions were partially prevented by DPI. Moreover, H2O2-induced release of IL-9, TNF-α, MIP-1α and MIP-1β was not counteracted by DPI, whereas no effect was observed in any experimental condition for both IL-6 and IL-1β. Our results support the view that ANP can play a key role during the inflammatory process.

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