Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2006198 | Peptides | 2013 | 11 Pages |
Abstract
Studies in mammals indicate that leptin is a multifunctional cytokine involved in regulation of energy metabolism and the modulation of the immune function. However, evidence for an immunomodulatory effect of leptin in fish is still missing. At least in part, this lack of knowledge is due to the absence of materials and models. In this study, we produced trout recombinant leptin (rt-lep) and tested its capacity to trigger cellular pathways, usually active in mammal immune system cells. STAT3, NF-κB, and the three major MAPK cascades (JNK, p38 and ERK), were activated by rt-lep in in vitro incubations with blood leucocytes of the rainbow trout Oncorhynchus mykiss. We also showed that rt-lep causes a decrease in superoxide anion production in trout blood leucocytes. Thus our data indicate that as in mammals also in teleosts leptin plays pleiotropic activities. Importantly, its actions in fishes do not always conform to the picture emerging for mammals.
Keywords
NF-κBIL-12CD4CD8ERKIL-6Jnkc-Jun N-terminal kinaseERK1/2MAPKp38 MAP kinasesuperoxide anioninterleukin 12interleukin 6tumor necrosis factor alphaCluster of differentiation 4SignalingTNF-αnuclear factor kappa-light-chain-enhancer of activated B cellsLeptinextracellular signal-regulated kinase 1/2extracellular-signal-regulated kinasemitogen-activated protein kinases
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Authors
Giovanna Mariano, Romania Stilo, Giuseppe Terrazzano, Elena Coccia, Pasquale Vito, Ettore Varricchio, Marina Paolucci,