Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2006375 | Peptides | 2010 | 7 Pages |
Abstract
ⶠLipopolysaccharide (LPS) plays a critical role in the pathogenesis of sepsis. ⶠA peptide (CLP19) derived from limulus anti-LPS factor (LALF) antagonized LPS significantly both in vitro and in vivo, but it displayed unnegligible hemolytic effect on human red blood cells. ⶠBy amino acid substitution, two new peptides (CLP19-1 and CLP19-2) were obtained from CLP19 and their bioactivities were evaluated. ⶠCLP19-1 exhibited equivalent LPS-detoxifying activity and much lower hemolysis compared with CLP19, while CLP19-2 showed little anti-LPS and cytotoxicity activities. ⶠThe changes in activities were probably attributable to the decrease of peptide hydrophobicity affected by substituting the hydrophobic residues by hydrophilic ones.
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Authors
Jiandong Ren, Hongfu Gao, Min Tang, Jinsong Gu, Peiyuan Xia, Guangxia Xiao,