Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2006398 | Peptides | 2011 | 7 Pages |
Abstract
A new bioactive tetradecapeptide, GFKDNVSNRIAHGFamide, was isolated from the brain of the squid, Todarodes pacificus. Using isolated T. pacificus esophagus as a bioassay, the peptide was shown to induce potent contraction of smooth muscle. The threshold concentration for contraction was 5Â ÃÂ 10â10Â M to 1Â ÃÂ 10â9Â M. The peptide was homologous to other molluskan (class Gastropoda) and annelid myoactive tetradecapeptides and to some extent, to arthropodan tridecapeptides. A full-length cDNA encoding the biosynthetic precursor of the active peptide was cloned, revealing that the peptide is probably secreted following processing of a prepropeptide containing a signal peptide and prosequences. This is the first myoactive tetradecapeptide (MATP) to be isolated from any mollusk of the class Cephalopoda and we have named it Todarodes tetradecapeptide (TTP).
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Authors
Hye-Jin Go, Eun Hee Jo, Jung-Kil Seo, Yong-Ki Hong, Hyung Ho Lee, Gun Do Kim, Tae Wook Park, Edward J. Noga, Nam Gyu Park,